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KMID : 0379520060220040403
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한국독성학회지
2006 Volume.22 No. 4 p.403 ~ p.409
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Neurotropism and Expression Pattern of lacZ Inserted PRV-Bartha in Geniculohypothalamic Tract Tracing
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Kim Jin-Sang
Park Eun-Se
Cheon Song-Hee
Kim Min-Hee
Bang Hyun-Soo
Kwon Young-Shil
Lee Bong-Hee
Kim Young-Chul
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Abstract
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To localize the connection between intergeniculate nucleus and suprachisasmatic nucleus through geniculohypothalamic tract in postnatal mongolian gerbil, we injected lacZ inserted PRV-Bartha strain into suprachiasrnatic nucleus and tried to immunostain against it with Rb134 and mouse anti-{beta}-galactosidase. The numbers of immunoreactive neurons in intergeniculate leaflet were 8{pm}3.2 in P1 Period, 10{pm}4.1 in P3 Period and 13{pm}6.2 in P7 Period, and was statistically significant (p<0.05) and had tendency to increase with time consuming. The results showed that intergeniculate leaflet had projected some axons into suprachiasrnatic nucleus through geniculohyptothalamic tract in postnatal mongolian gerbil. But we could not exclude the possibility of direct projections from dorsal and ventral geniculate nuclei into suprachisamatic nucleus completely.
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KEYWORD
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Intergeniculate leaflet, Suprachiasmatic nucleus, geniculohypothalamic tract, lacZ, PRV-Bartha, Immuostain
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